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The eluting substances had been cryogenically accumulated on a spirally rotating ZnSe disk cooled by liquid nitrogen to −40°C. IR spectra have been recorded in the IR‐clear ZnSe disk utilizing a nitrogen‐cooled mercury cadmium telluride (MCT) detector.
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6‐allyl radical, which consequently advised that the hydroxylation couldn't have occurred on the allyl team. As demonstrated in Figure 2b, the radical lack of the N
The ESI‐QTOF tandem mass spectrum recorded for 1P‐AL‐LAD immediately after incubation with pHLM is proven in Figure 2b (proposed fragmentation pathways during the supporting details). The bottom peak was detected at m/z
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Fourteen metabolites ended up detected in whole; metabolic reactions bundled hydroxylation on the Main lysergamide ring construction or the N
As revealed inside the supporting information, the detection of AL‐LAD while in the pHLM‐good incubation mixture verified its development to a major extent significantly beyond the incredibly reduced abundance present in the pHLM‐destructive sample.
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